E carboxylterminal region of phospholipase C-1 by calpain abolishes activation by Gq. J Biol Chem. 1993;268:3710?. 16. Kim CG, Park D, Rhee SG. The role of carboxyl-terminal basic amino acids in Gq-dependent activation, particulate association, and nuclear localization of phospholipase C-1. J Biol Chem. 1996;271:21187?2. 17. Venkatakrishnan G, Exton J. Identification of determinants in the -subunit of Gq required for phospholipase C activation. J Biol Chem. 1996;271:5066?2. 18. Wilkie TM, Scherle PA, Strathmann MP, Slepak VZ, Simon MI. Characterization of G-protein subunits in the Gq class: expression in murine tissues and in stromal and hematopoietic cell lines. Proc Natl Acad Sci. 1991;88(22):10049?3. 19. Wilkie TM, Gilbert DJ, Olsen AS, Chen XN, Amatruda TT, Korenberg JR, et al. Evolution of the mammalian G protein subunit multigene family. Nat Genet. 1992;1(2):85?1. 20. Conklin BR, Chabre O, Wong YH, Federman A, Bourne HR. Recombinant Gq: mutational activation and coupling to receptors and phospholipase C. J Biol Chem. 1992;267:31?. 21. Hepler JR, Kozasa T, Smrcka AV, Simon MI, Rhee SG, Sternweis PC, et al. Purification from Sf9 cells and characterization of recombinant Gq and G11. Activation of purified phospholipase C isozymes by G subunits. J Biol Chem. 1993;268(19):14367?5.22. Mody SM, Ho MK, Joshi SA, Wong YH. Incorporation of Gz-specific sequence at the carboxyl terminus increases the promiscuity of G16 toward Gi-coupled receptors. Mol Pharmacol. 2000;57:13?3. 23. Tian YJ, New DC, Yung LY, Allen RA, Slocombe PM, Twomey BM, et al. Differential chemokine activation of CCR1-regulated pathways: selective activation of G14-coupled pathways. Eur J Immunol. 2004;34:785?5. 24. Chan JSC, Lee JWM, Ho MKC, Wong YH. Preactivation permits subsequent stimulation of phospholipase C by Gi-coupled receptors. Mol Pharmacol. 2000;57:700?. 25. Liu AMF, Lo RKH, Guo EX, Ho MKC, Ye RD, Wong YH. G16 interacts with tetratricopeptide repeat 1 (TPR1) through its 3 region to activate Ras independently of phospholipase C signaling. BMC Struct Biol. 2011;11:17. 26. Tsu RC, Ho MK, Yung LY, Joshi S, Wong YH. Role of amino- PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/18111632 and carboxylterminal regions of Gz in the recognition of Gi-coupled receptors. Mol Pharmacol. 1997;52(1):38?5. 27. Ho MK, Wong YH. The amino terminus of Gz is required for receptor recognition, whereas its 4/6 loop is essential for inhibition of adenylyl cyclase. Mol Pharmacol. 2000;58:993?000. 28. Ho MK, Chan JH, Wong CS, Wong YH. Identification of a stretch of six divergent amino acids on the 5 helix of G 16 as a major determinant of the promiscuity and efficiency of receptor coupling. Biochem J. 2004;380(Pt 2):361?. 29. Lo RKH, Wong YH. Signal transducer and activator of transcription 3 activation by the -opioid receptor via G14 involves multiple intermediates. Mol Pharmacol. 2004;65:1427?9. 30. Yung LY, Joshi SA, Chan RYK, Chan JSC, Pei G, Wong YH. GL1 (G14) couples the ORL1 receptor to stimulation of phospholipase C. J Pharmacol Exp Ther. 1999;288:232?. 31. Liu W, Northup JK. The helical domain of a G protein subunit is a regulator of its effector. Proc Natl Acad Sci U S A. 1998;95:12878?3. 32. Lee CH, Park D, Wu D, Rhee Letrozole SG, Simon MI. Members of the Gq subunit PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9547713 gene family activate phospholipase C isozymes. J Biol Chem. 1992;267:16044?. 33. Taylor S, Chae H, Rhee S, Exton J. Activation of the 1 isozyme of phospholipase C by subunits of the Gq class of G proteins. Nature. 1991;350:516?. 34. Kwan DHT, Yung LY, Ye RD, Wong YH. Activ.